NGS library preparation. Evolved.
Evolved to Focus.
your qPCR inhibitions
Library preparation conditions were optimized to successfully reduce DNA sample input from 200 ng (the minimum input according to the manufacturer’s protocol) to “10 ng” by modifying SureSelect libraries for target enrichment through combination with the KAPA Hyper Prep Kit. This workflow enabled targeted deep sequencing without increasing PCR duplicates in NGS. Read more
The KAPA Hyper PCR-free system appears to be the best solution for specimens in which ∼500 ng of high-quality DNA is available for library preparation. Read more
Learn how the Hyper Prep Kit and Kapa HiFi Uracil PCR Polymerase were used to evaluate whether CpG methylation of transposable elements are associated with anaplastic lymphoma kinase expression in cancer. Read More
Researchers at Foundation Medicine have created a test to interrogate base substitutions, indels, copy number alterations and selected fusions across 287 cancer related genes from FFPE tumor samples using massively parallel next generation sequencing. Read More
Researchers at the Broad Institute evaluate four sequencing platforms using human and microbial samples to assess sources of bias in sequencing and sample preparation workflow processes. Read More
Researchers at the Sanger Center investigate many thermostable DNA polymerases along with various reaction conditions for adapter-ligated fragments for Illumina Sequencing in efforts to determine and reduce bias. Read More
I would recommend the KAPA hgDNA Quantification and QC Kit to all CAP/CLIA labs and cancer testing centers because it is the most robust solution for screening both quantity and quality of sample material.